{"id":1179,"date":"2024-10-01T07:00:58","date_gmt":"2024-09-30T22:00:58","guid":{"rendered":"https:\/\/rakudaseitai.com\/?p=1179"},"modified":"2025-06-13T07:16:26","modified_gmt":"2025-06-12T22:16:26","slug":"edna_method","status":"publish","type":"post","link":"https:\/\/rakudaseitai.com\/?p=1179","title":{"rendered":"\u74b0\u5883DNA\u5206\u6790\u624b\u6cd5\u306e\u6982\u8981"},"content":{"rendered":"<p>\u74b0\u5883DNA\u5206\u6790\u624b\u6cd5\u306f\u3001<strong>\u2460\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u30fb\u308d\u904e\uff08DNA\u306e\u6fc3\u7e2e\uff09\u3001\u2461\u74b0\u5883DNA\u306e\u62bd\u51fa\u3001\u2462\u74b0\u5883DNA\u306e\u62bd\u51fa<\/strong>\u306e3\u3064\u306e\u5de5\u7a0b\u306b\u5927\u5225\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p class=\"hh 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class=\"hh hh1\"><strong>\u74b0\u5883DNA\u306e\u62bd\u51fa<\/strong><\/p>\n<p>DNA\u306e\u62bd\u51fa\u306fPCR\u306a\u3069\u3068\u7570\u306a\u308a\u3084\u308a\u76f4\u3057\u304c\u3067\u304d\u305a\u3001\u62bd\u51fa\u306e\u7d50\u679c\u6b21\u7b2c\u3067\u305d\u308c\u4ee5\u964d\u306e\u5206\u6790\u306b\u5927\u304d\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3059\u305f\u3081\u3001\u7279\u306b\u6ce8\u610f\u304c\u5fc5\u8981\u306a\u4f5c\u696d\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u74b0\u5883DNA\u3092\u62bd\u51fa\u3059\u308b\u65b9\u6cd5\u3068\u3057\u3066\u3001\u73fe\u5728\u591a\u304f\u4f7f\u308f\u308c\u3066\u3044\u308b\u624b\u6cd5\u306f\u3001DNeasy Blood \uff06 Tissue Kit \u3092\u7528\u3044\u305f\u62bd\u51fa\u6cd5\u3067\u3001\u65e5\u672c\u306e\u74b0\u5883DNA\u5206\u6790\u306b\u304a\u3044\u3066\u3082\u3053\u306e\u30ad\u30c3\u30c8\u304c\u4e3b\u306b\u7528\u3044\u3089\u308c\u3066\u304d\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p class=\"hh hh1\"><strong>\u74b0\u5883DNA\u306e\u691c\u51fa\u65b9\u6cd5<\/strong><\/p>\n<p>\u74b0\u5883DNA\u306e\u691c\u51fa\u306b\u306f\u4e3b\u306b\u3001<\/p>\n<p>\u2460\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u304a\u3088\u3073\u96fb\u6c17\u6cf3\u52d5<\/p>\n<p>\u2461\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR<\/p>\n<p>\u2462\u30c7\u30b8\u30bf\u30ebPCR<\/p>\n<p>\u2463\u8d85\u4e26\u5217\u30b7\u30fc\u30b1\u30f3\u30b9<\/p>\n<p>\u306e4\u3064\u306e\u65b9\u6cd5\u304c\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u2460\uff5e\u2462\u306f\u57fa\u672c\u7684\u306b\u5358\u4e00\u306e\u7a2e\u3092\u5bfe\u8c61\u3068\u3057\u305f\u7a2e\u7279\u7570\u691c\u51fa\u3001\u2463\u306f\u7279\u5b9a\u306e\u5206\u985e\u7fa4\uff08\u9b5a\u985e\u306a\u3069\uff09\u3092\u307e\u3068\u3081\u3066\u691c\u51fa\u3059\u308b\u7db2\u7f85\u7684\u691c\u51fa\u3068\u547c\u3070\u308c\u307e\u3059\u3002\u3044\u305a\u308c\u306e\u691c\u51fa\u6cd5\u3082\u5bfe\u8c61\u7a2e\uff08\u3082\u3057\u304f\u306f\u5bfe\u8c61\u5206\u985e\u7fa4\uff09\u306b\u5408\u308f\u305b\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u304a\u3088\u3073\u4f7f\u7528\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u7a2e\u7279\u7570\u691c\u51fa\u306f\u3001\u7279\u5b9a\u306e\u7a2e\u3092\u5bfe\u8c61\u3068\u3057\u3001\u305d\u306e\u7a2e\u306eDNA\u304c\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u542b\u307e\u308c\u308b\u304b\u3069\u3046\u304b\u3001\u3082\u3057\u304f\u306f\u3069\u308c\u304f\u3089\u3044\u306e\u91cf\u304c\u3042\u308b\u304b\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u307e\u3059\u3002<\/p>\n<p><strong>\u2460\uff5e\u2462\u306e\u3069\u306e\u624b\u6cd5\u3067\u3082DNA\u3092\u5b9a\u6027\u7684\u306b\u691c\u51fa\uff08\u5728\u30fb\u4e0d\u5728\uff09\u3067\u304d\u307e\u3059\u304c\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3082\u3057\u304f\u306f\u30c7\u30b8\u30bf\u30ebPCR\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001DNA\u306e\u5b9a\u91cf\u7684\u306a\u691c\u51fa\u3082\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002<\/strong><\/p>\n<p>\u7db2\u7f85\u7684\u691c\u51fa\u306f\u3001\u3042\u308b\u5bfe\u8c61\u5206\u985e\u7fa4\u3092\u307e\u3068\u3081\u3066\u5897\u5e45\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\uff08\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\uff09\u3068\u8d85\u4e26\u5217\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u5bfe<strong>\u8c61\u3068\u306a\u308b\u5206\u985e\u7fa4\u306b\u5c5e\u3059\u308b\u7a2e\u3092\u7db2\u7f85\u7684\u306b\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/strong>\u305d\u306e\u305f\u3081\u3001\u8abf\u67fb\u5730\u306b\u3069\u306e\u3088\u3046\u306a\u9b5a\u985e\u304c\u751f\u606f\u3057\u3066\u3044\u308b\u304b\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u306a\u3069\u306b\u7528\u3044\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u624b\u6cd5\u306f\u7a2e\u7279\u7570\u691c\u51fa\u306b\u6bd4\u3079\u3066\u3001\u5b9a\u91cf\u7684\u306a\u30c7\u30fc\u30bf\u3092\u5f97\u308b\u3053\u3068\u306f\u56f0\u96e3\u3067\u3059\u304c\u3001\u8fd1\u5e74\u5b9a\u91cf\u7684\u6d3b\u7db2\u7f85\u7684\u306a\u89e3\u6790\u306e\u8a66\u307f\u3068\u3057\u3066\u3001\u30e9\u30f3\u30c0\u30e0\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u3088\u308b\u78ba\u7387\u7684\u30e9\u30d9\u30ea\u30f3\u30b0\u3092\u7528\u3044\u305f\u65b9\u6cd5\uff08Hoshino \uff06 Inagaki, 2017\uff09\u3084\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9DNA\u3092\u6dfb\u52a0\u3059\u308b\u65b9\u6cd5\uff08Ushio et al., 2018\uff09\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p class=\"hh hh2 main-c main-bdr\">\u2460PCR\u3068\u96fb\u6c17\u6cf3\u52d5\u3092\u7528\u3044\u305f\u74b0\u5883DNA\u306e\u691c\u51fa<\/p>\n<p>\u3053\u306e\u624b\u6cd5\u306f\u3001\u30ea<strong>\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3084\u30c7\u30b8\u30bf\u30ebPCR\u3092\u7528\u3044\u305f\u65b9\u6cd5\u3088\u308a\u3082\u5b89\u4fa1\u306b\u5b9f\u65bd\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/strong><\/p>\n<p>\u4e00\u65b9\u3001\u5f97\u3089\u308c\u308b\u30c7\u30fc\u30bf\u306f\u96fb\u6c17\u6cf3\u52d5\u3067\u78ba\u8a8d\u3055\u308c\u308b\u30d0\u30f3\u30c9\u306e\u6709\u7121\u304b\u3089\u5224\u65ad\u3055\u308c\u308b\u5bfe\u8c61\u7a2e\u306e\u5728\u30fb\u4e0d\u5728\u30c7\u30fc\u30bf\u3067\u3042\u308a\u3001\u7e70\u308a\u8fd4\u3057\u6570\u3084\u30d0\u30f3\u30c9\u306e\u6fc3\u6de1\u304b\u3089\u5224\u5b9a\u91cf\u7684\u306a\u60c5\u5831\u304c\u5f97\u3089\u308c\u308b\u5834\u5408\u3082\u3042\u308b\u3082\u306e\u306e\u3001\u5b9a\u91cf\u6027\u306f\u307b\u307c\u306a\u3044\u3068\u898b\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u96fb\u6c17\u6cf3\u52d5\u6642\u3001\u9ad8\u6fc3\u5ea6\u306e\u6a19\u7684\u914d\u5217\u3092\u542b\u3080PCR\u7523\u7269\u3092\u6271\u3046\u969b\u306b\u3001\u4ed6\u306e\u30b5\u30f3\u30d7\u30eb\u3084\u5b9f\u9a13\u5668\u5177\u3092\u6c5a\u67d3\u3057\u3066\u3057\u307e\u3046\u5371\u967a\u304c\u3042\u308a\u3001\u6ce8\u610f\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p 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